Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,274,854	G→A	T108M (ACG→ATG)	ychN ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,274,854	0	G	A	100.0%	116.8 / NA	37	T108M (ACG→ATG)	ychN	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/11);  total (26/11)

GCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCC  >  W3110S.gb/1274801‑1274893                                                      |                                        gCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc                                       >  1:1011809/1‑56 (MQ=255) gCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc                                       >  1:1163924/1‑56 (MQ=255) gCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc                                       >  1:4362719/1‑56 (MQ=255) gCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc                                       >  1:3718956/1‑56 (MQ=255) gCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATc                                       >  1:3578364/1‑56 (MQ=255)           cccccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCca                       <  1:608319/62‑1 (MQ=255)           cccccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCca                       <  1:3327354/62‑1 (MQ=255)             cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTcc                        >  1:4343925/1‑59 (MQ=255)             cccTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTcc                        >  1:3181024/1‑59 (MQ=255)               cTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAag                   <  1:942791/62‑1 (MQ=255)               cTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAag                   <  1:4536298/62‑1 (MQ=255)               cTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAag                   <  1:1621340/62‑1 (MQ=255)                tCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAag                   >  1:103766/1‑61 (MQ=255)                tCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAag                   >  1:2768096/1‑61 (MQ=255)                 cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:1981594/59‑1 (MQ=255)                 cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:4662048/59‑1 (MQ=255)                 cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:1510861/59‑1 (MQ=255)                 cAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:2584455/59‑1 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATGCATTgc                                 >  1:3253139/1‑39 (MQ=37)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:3801606/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:874616/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:1135332/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:4700149/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:450213/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:1421363/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:3532739/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:2436056/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:2832096/1‑59 (MQ=255)                        tAAAATGTGAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACAAGAGTAcc             >  1:2804188/1‑59 (MQ=255)                             tgtgAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:1296945/47‑1 (MQ=255)                              gtgAGCACTTTATCGGCTGACAGCATCCATTGCGCCAGTTCCACaa                    <  1:2599695/46‑1 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTc       >  1:3511951/1‑42 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc  >  1:3873109/1‑47 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc  >  1:4130181/1‑47 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc  >  1:3605277/1‑47 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc  >  1:4380988/1‑47 (MQ=255)                                               tGACAGCATCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCAccc  >  1:1179800/1‑47 (MQ=255)                                                      |                                        GCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCC  >  W3110S.gb/1274801‑1274893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑