Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	275,785	G→A	V413I (GTA→ATA)	mmuP →	predicted S‑methylmethionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	275,785	0	G	A	100.0%	178.4 / NA	55	V413I (GTA→ATA)	mmuP	predicted S‑methylmethionine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

AGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAG  >  W3110S.gb/275730‑275839                                                        |                                                       aGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATAtt                                                    >  1:2004432/1‑60 (MQ=255) aGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATAtt                                                    >  1:2883342/1‑60 (MQ=255)            tGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:2455203/59‑1 (MQ=255)             gAGTGCATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:4281609/58‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:1112251/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:4231946/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:3683407/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:1551974/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:4520469/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:1816616/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgct                                        <  1:2614221/60‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:2415985/58‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:2158304/58‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:1748297/58‑1 (MQ=255)             gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:3232363/58‑1 (MQ=255)               gTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg                                          <  1:482467/56‑1 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:4237664/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:4639269/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:437501/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:11507/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:1473035/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGcc                                   >  1:1597819/1‑58 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:1996349/62‑1 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:1248337/62‑1 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:3141065/62‑1 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:2694196/62‑1 (MQ=255)                    ttACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:2663023/62‑1 (MQ=255)                     tccATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:4284432/59‑1 (MQ=255)                     tACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:4601381/61‑1 (MQ=255)                     tACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:2992419/61‑1 (MQ=255)                           aTCGCGCGCCGTGGTATCCGCTGGTGGCAATATTAGGTTTTGTGCTGTGCCtggt                               <  1:4400843/55‑1 (MQ=255)                             cgcgcgCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTgtgt                      >  1:194035/1‑62 (MQ=255)                             cgcgcgCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTgtgt                      >  1:2184592/1‑62 (MQ=255)                              gcgcgcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGtt                     <  1:683068/62‑1 (MQ=255)                              gcgcgcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGtt                     <  1:3866344/62‑1 (MQ=255)                                  gcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc                 >  1:2790269/1‑62 (MQ=255)                                  gcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc                 >  1:2486523/1‑62 (MQ=255)                                  gcCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc                 >  1:4196780/1‑62 (MQ=255)                                  gcCGTGGTATCCGCTGCTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc                 >  1:2492086/1‑62 (MQ=255)                                     gTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTgg              <  1:1166098/62‑1 (MQ=255)                                     gTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGGGCCTGGTGGCCTGTGTTGGGCTgg              <  1:3166682/62‑1 (MQ=255)                                     gTGGTATCCGCTGGTGCCAATATTAGGTTTTGAGCTGTGCCTGGTGGCCTGTGTTGGGCTgg              <  1:1939706/62‑1 (MQ=255)                                               cTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATcc    >  1:964957/1‑62 (MQ=255)                                               cTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATcc    >  1:4318987/1‑62 (MQ=255)                                               cTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATcc    >  1:2888072/1‑62 (MQ=255)                                               cTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATc     >  1:506884/1‑61 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGtt                     >  1:835006/1‑40 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:33640/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:3136359/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:2425069/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:4644562/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:215751/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:581790/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:1549176/1‑59 (MQ=255)                                                    gCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg  >  1:1137485/1‑59 (MQ=255)                                                        |                                                       AGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAG  >  W3110S.gb/275730‑275839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑