Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,303,633	G→T	Q246Y (CAG→TAT)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,303,633	0	G	T	100.0%	141.6 / NA	43	Q246Y (CAG→TAT)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/24);  total (19/24)

TCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCT  >  W3110S.gb/1303582‑1303679                                                    |                                               tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:3622758/1‑62 (MQ=255) tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:1115940/1‑62 (MQ=255) tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:2977009/1‑62 (MQ=255) tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:153789/1‑62 (MQ=255) tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:4338440/1‑62 (MQ=255) tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                      >  1:561765/1‑62 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                            <  1:2076405/55‑1 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                            <  1:4157045/55‑1 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                            <  1:4208019/55‑1 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                            <  1:281111/55‑1 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                            <  1:1843308/55‑1 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                     >  1:3779411/1‑62 (MQ=255)  cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                     >  1:2475601/1‑62 (MQ=255)       aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                   <  1:871350/59‑1 (MQ=255)       aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                   <  1:2951137/59‑1 (MQ=255)         gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                              <  1:3699511/62‑1 (MQ=255)         gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                              <  1:4585842/62‑1 (MQ=255)         gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                              <  1:2187678/62‑1 (MQ=255)         gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                              <  1:4451472/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:690609/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:69808/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:1193792/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:1396946/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:1473387/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:1665015/62‑1 (MQ=255)           aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                            <  1:1936278/62‑1 (MQ=255)                 aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                      <  1:1019371/62‑1 (MQ=255)                 aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                      <  1:620212/62‑1 (MQ=255)                 aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                      <  1:3423186/62‑1 (MQ=255)                 aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                      <  1:1591824/62‑1 (MQ=255)                 aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                      <  1:1013596/62‑1 (MQ=255)                      cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                 >  1:35483/1‑62 (MQ=255)                      cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                 >  1:2848216/1‑62 (MQ=255)                         gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                   <  1:4677632/41‑1 (MQ=38)                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa             >  1:52636/1‑62 (MQ=255)                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa             >  1:1024978/1‑62 (MQ=255)                             aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg           >  1:3044370/1‑61 (MQ=255)                             aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg           >  1:2771090/1‑61 (MQ=255)                             aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg           >  1:4422490/1‑61 (MQ=255)                             aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg           >  1:2152791/1‑61 (MQ=255)                             aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg           >  1:1644759/1‑61 (MQ=255)                                     aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt  >  1:254234/1‑62 (MQ=255)                                     aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt  >  1:1592548/1‑62 (MQ=255)                                                    |                                               TCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCT  >  W3110S.gb/1303582‑1303679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑