Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,364,882	G→A	V142V (GTG→GTA)	puuC →	gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,364,882	0	G	A	100.0%	244.6 / NA	73	V142V (GTG→GTA)	puuC	gamma‑Glu‑gamma‑aminobutyraldehyde dehydrogenase, NAD(P)H‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/44);  total (29/44)

CGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGT  >  W3110S.gb/1364825‑1364929                                                          |                                                cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:3290684/1‑61 (MQ=255) cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:4164548/1‑61 (MQ=255) cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:1343042/1‑61 (MQ=255) cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:4187738/1‑61 (MQ=255) cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:472979/1‑61 (MQ=255) cgcggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCg                                              >  1:2000760/1‑61 (MQ=255)    ggcgcgCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGacca                                          <  1:3194791/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:4492013/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:3025216/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2869472/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:3936537/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:3949892/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:245818/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2284799/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2040281/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:4680187/62‑1 (MQ=255)     gcgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:955348/62‑1 (MQ=255)      cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:3623573/61‑1 (MQ=255)      cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2069957/61‑1 (MQ=255)      cgcgcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGGGTATGGCGAAGTAGCGaccac                                         <  1:3340715/61‑1 (MQ=255)         gcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGt                                     <  1:1341738/62‑1 (MQ=255)         gcgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGt                                     <  1:3099543/62‑1 (MQ=255)          cgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGt                                     <  1:4690803/61‑1 (MQ=255)          cgcCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGt                                     <  1:2132005/61‑1 (MQ=255)                    tGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCt                          <  1:4539300/62‑1 (MQ=255)                    tGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCt                          <  1:859238/62‑1 (MQ=255)                    tGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCt                          <  1:1170996/62‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAATTGTATGGCGAAGTAGCGaccac                                         <  1:3250645/44‑1 (MQ=38)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1115098/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2661733/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2612920/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1733268/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1088632/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1288008/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2449601/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2435830/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1354958/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2384536/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:4362083/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2309342/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2308217/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:788172/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:67019/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:2038583/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:200566/44‑1 (MQ=255)                       tACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:1990475/44‑1 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGaccac                                         <  1:4117829/43‑1 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:4108233/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:939815/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2504430/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:3123107/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:114427/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1193697/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1286096/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1666460/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1667987/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1900629/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1953148/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2352750/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2420013/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2428545/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:1070388/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2564869/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2574477/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2754068/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:2991248/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:3117653/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCg                      >  1:319050/1‑62 (MQ=255)                        aCGCCGAAGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACAAGTAGCCATGAGCTGGCg                      >  1:958288/1‑62 (MQ=255)                               aGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcg               <  1:2231234/62‑1 (MQ=255)                               aGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcg               <  1:402400/62‑1 (MQ=255)                               aGCGATCGACAAAGTGTATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATcg               <  1:1683529/62‑1 (MQ=255)                                            gtgtATGGCGAAGTAGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGt  >  1:1713193/1‑62 (MQ=255)                                                          |                                                CGCGGCGCGCGCCATTCGCTGGTACGCCGAAGCGATCGACAAAGTGTATGGCGAAGTGGCGACCACCAGTAGCCATGAGCTGGCGATGATCGTGCGTGAACCGGT  >  W3110S.gb/1364825‑1364929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑