Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,393,837	C→T	R45* (CGA→TGA)	ycjZ →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,393,837	0	C	T	100.0%	53.5 / NA	17	R45* (CGA→TGA)	ycjZ	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/0);  total (17/0)

CTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGA  >  W3110S.gb/1393786‑1393889                                                    |                                                     cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGAtt                                            >  1:3564774/1‑62 (MQ=255)               gTCAGCTTTAAGCCAGATAGTGCGACGTATAGAAGAATGATTGGGATTGCGGCTTCTGAcg                               >  1:721418/1‑61 (MQ=255)                 cAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGa                            >  1:2396267/1‑62 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAAcc                         >  1:3114807/1‑57 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcg                      >  1:1522928/1‑60 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcg                      >  1:1999942/1‑60 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcg                      >  1:243942/1‑60 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcg                      >  1:2890879/1‑60 (MQ=255)                         aGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAAACAcg                      >  1:2740941/1‑60 (MQ=255)                             aGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGc                >  1:2293567/1‑62 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCt                                   >  1:2426839/1‑37 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt           >  1:1267068/1‑61 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt           >  1:3679119/1‑61 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt           >  1:4563804/1‑61 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt           >  1:1881068/1‑61 (MQ=255)                                   tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt           >  1:812629/1‑61 (MQ=255)                                           ataGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGa  >  1:956316/1‑62 (MQ=255)                                                    |                                                     CTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGA  >  W3110S.gb/1393786‑1393889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑