Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,413,523	G→A	S47F (TCC→TTC)	ydaO ←	predicted C32 tRNA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,413,523	0	G	A	100.0%	107.5 / NA	33	S47F (TCC→TTC)	ydaO	predicted C32 tRNA thiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/24);  total (9/24)

ATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTC  >  W3110S.gb/1413480‑1413581                                            |                                                           aTTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGc                                          <  1:1713751/62‑1 (MQ=255)                     tGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata                      >  1:1028264/1‑62 (MQ=255)                     tGGTATAGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCata                      >  1:80034/1‑62 (MQ=255)                         ataGCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:4210917/62‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATTATGCGATCGCCTTCTTCAAt                          <  1:4252669/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:1166670/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:739718/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:63337/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:4303190/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:4247221/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:4133198/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:3975416/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:2870115/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:2329011/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:226967/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:1517035/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:1521716/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:157292/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:165657/51‑1 (MQ=255)                            gCTGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAAt                          <  1:180873/51‑1 (MQ=255)                              tGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTc              >  1:3834161/1‑61 (MQ=255)                              tGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTc              >  1:434514/1‑61 (MQ=255)                              tGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTc              >  1:3384421/1‑61 (MQ=255)                              tGTCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTc              >  1:3215530/1‑61 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:2179297/55‑1 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:4224231/55‑1 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:1754214/55‑1 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:1690588/55‑1 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:4329628/55‑1 (MQ=255)                                tCTTTACCCCCGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGa                  <  1:4517093/55‑1 (MQ=255)                                         ccGAAGAGGCAAACCATGATGCGATCGCCTTTTTCAATCATATTGAAGTCAGCAATGGCTTc  >  1:3455236/1‑62 (MQ=255)                                         ccGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTc  >  1:1977786/1‑62 (MQ=255)                                         ccGAAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTc  >  1:2273039/1‑62 (MQ=255)                                            |                                                           ATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTC  >  W3110S.gb/1413480‑1413581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑