Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,516,711	C→T	A79V (GCG→GTG)	ydcV →	predicted spermidine/putrescine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,516,711	0	C	T	100.0%	108.6 / NA	33	A79V (GCG→GTG)	ydcV	predicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/9);  total (24/9)

TGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAA  >  W3110S.gb/1516661‑1516768                                                   |                                                          tGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCTACATTAATTCTGCTGGTGTTa                                                 >  1:3210869/1‑61 (MQ=38) tGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTa                                                 >  1:2384674/1‑61 (MQ=255) tGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTa                                                 >  1:341723/1‑61 (MQ=255) tGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTATGCTGGTGTTa                                                 >  1:1127130/1‑61 (MQ=255) tGATGCCGTGACACTGTCACTTAAAGTGGAGGCGCTGGCGACATTAATTGTGCTGGTGTTa                                                 >  1:3691310/1‑61 (MQ=255)   aTGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAggg                                              >  1:2153496/1‑62 (MQ=255)    tGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAg                                                <  1:1733657/59‑1 (MQ=255)    tGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGa                                             >  1:3572899/1‑62 (MQ=255)    tGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGa                                             >  1:1374067/1‑62 (MQ=255)    tGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGa                                             >  1:1446529/1‑62 (MQ=255)      ccGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACg                                           >  1:2627899/1‑62 (MQ=255)                gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTg                                 <  1:3294619/62‑1 (MQ=255)                gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTg                                 <  1:4543217/62‑1 (MQ=255)                       agaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:2545036/3‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTTCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:3947891/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGAc                                            >  1:3436997/1‑44 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGAc                                            >  1:3564250/1‑44 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGAc                                            >  1:1470055/1‑44 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGAc                                            >  1:786807/1‑44 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCgcgc                           >  1:3128321/1‑61 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:4303229/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:4545769/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:991521/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:3566092/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:2248390/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:2077029/1‑62 (MQ=255)                       aaaGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt                          >  1:135549/1‑62 (MQ=255)                                       cGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTg                                 <  1:1106984/39‑1 (MQ=255)                                              aaTTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCa   <  1:1111495/62‑1 (MQ=255)                                                ttGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaa  <  1:2029293/61‑1 (MQ=255)                                                ttGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaa  <  1:1706736/61‑1 (MQ=255)                                                ttGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaa  <  1:1238148/61‑1 (MQ=255)                                                ttGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaa  <  1:694131/61‑1 (MQ=255)                                                   |                                                          TGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAA  >  W3110S.gb/1516661‑1516768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑