Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	285,498	G→A	A294T (GCC→ACC)	yagG →	predicted sugar transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	285,498	0	G	A	100.0%	244.9 / NA	76	A294T (GCC→ACC)	yagG	predicted sugar transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/40);  total (36/40)

ACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGA  >  W3110S.gb/285441‑285543                                                          |                                              accaTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCtt                                           <  1:1938617/62‑1 (MQ=255)  ccaTGTTCGGCGCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCtt                                           <  1:170832/61‑1 (MQ=255)     tGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAg                                       <  1:3206952/62‑1 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGCCCGCGTCACCACCTTCAAGTg                                     >  1:3913358/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACTGCGTCACCACCTTCAAGTg                                     >  1:4011664/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGt                                      >  1:762065/1‑61 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:4628476/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:3428742/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:2314986/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:3854107/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     <  1:2963829/62‑1 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     <  1:4426529/62‑1 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:4689390/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:4439726/1‑62 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     <  1:1521866/62‑1 (MQ=255)       ttCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg                                     >  1:4485796/1‑62 (MQ=255)           gCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc                                 >  1:4692235/1‑62 (MQ=255)           gCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc                                 >  1:1138354/1‑62 (MQ=255)           gCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc                                 >  1:3503308/1‑62 (MQ=255)           gCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatc                                 >  1:1595622/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGttcatc                              >  1:1643364/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:2922436/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:3089457/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:859621/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:3363165/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:4002833/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:4648363/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:1087312/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:1598315/1‑62 (MQ=255)              cGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatcatc                              >  1:1563153/1‑62 (MQ=255)                 ttttCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2056171/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:27039/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:499557/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:4095474/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2186440/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:3980549/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2276791/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:581800/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2291062/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:713639/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:745965/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2519640/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:751347/60‑1 (MQ=255)                 tttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:3213751/60‑1 (MQ=255)                 gttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:4411119/59‑1 (MQ=255)                  ttGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:4311475/59‑1 (MQ=255)                             gCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcg                             <  1:2978431/48‑1 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCAGCTTCAAGTGGATCATCGTCGCCTa                      >  1:2564234/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATGATCGTCGCCTa                      >  1:3409917/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCAtcgtc                           >  1:2146043/1‑43 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCt                       >  1:788447/1‑47 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:1732291/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:1154813/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:4419661/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:4686845/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:4026752/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:2204309/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:3641783/1‑48 (MQ=255)                                    gggCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTa                      >  1:580839/1‑48 (MQ=255)                                          cTTCGATCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:4324899/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:518065/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:813276/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:106059/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:1232427/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:1296198/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:4222904/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3882685/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3627728/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3516270/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3440066/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:2370638/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3309090/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:2568302/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3086420/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:3075309/62‑1 (MQ=255)                                          cTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa  <  1:2619675/62‑1 (MQ=255)                                                          |                                              ACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGA  >  W3110S.gb/285441‑285543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑