Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,516,927	A→G	N151S (AAT→AGT)	ydcV →	predicted spermidine/putrescine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,516,927	0	A	G	100.0%	132.1 / NA	36	N151S (AAT→AGT)	ydcV	predicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/24);  total (12/24)

ATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCT  >  W3110S.gb/1516881‑1516984                                               |                                                          aTCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGttt                                            <  1:2241360/62‑1 (MQ=255) aTCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGttt                                            <  1:215679/62‑1 (MQ=255) aTCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGttt                                            <  1:3129387/62‑1 (MQ=255) aTCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGttt                                            <  1:1802396/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTGATCGCCCGTTTTcgccg                                      <  1:2735546/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1055193/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:2620883/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:3112387/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:2009660/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1912454/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1348785/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:3698785/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1231957/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1203814/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:3905927/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:1174665/62‑1 (MQ=255)       gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTcgccg                                      <  1:69613/62‑1 (MQ=255)           gtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcc                                  >  1:665930/1‑62 (MQ=255)              aTGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcc                               >  1:2507957/1‑62 (MQ=255)              aTGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcc                               >  1:1136419/1‑62 (MQ=255)              aTGCGACTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCAcctcc                               >  1:1181996/1‑62 (MQ=255)                   aCTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAg                          <  1:315462/62‑1 (MQ=255)                   aCTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAg                          <  1:2883/62‑1 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAg                          >  1:1073256/1‑61 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGt                         >  1:2752142/1‑62 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGt                         >  1:2144771/1‑62 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGt                         >  1:2150357/1‑62 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGt                         >  1:1346775/1‑62 (MQ=255)                    cTTTTTGTGTAGTTGTGGTGTTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGt                         >  1:2184596/1‑62 (MQ=255)                                      tgtTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATg       >  1:3951786/1‑62 (MQ=255)                                      tgtTTAACAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATg       >  1:252819/1‑62 (MQ=255)                                            aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt  <  1:1868703/61‑1 (MQ=255)                                            aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt  <  1:3566993/61‑1 (MQ=255)                                            aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt  <  1:2726964/61‑1 (MQ=255)                                            aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt  <  1:4097804/61‑1 (MQ=255)                                            aCAGTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCt  <  1:4677846/61‑1 (MQ=255)                                               |                                                          ATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCT  >  W3110S.gb/1516881‑1516984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑