Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,559,619	A→G	L45S (TTA→TCA)	ddpF ←	D‑Ala‑D‑Ala transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,559,619	0	A	G	100.0%	117.1 / NA	32	L45S (TTA→TCA)	ddpF	D‑Ala‑D‑Ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/23);  total (9/23)

AAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTT  >  W3110S.gb/1559568‑1559673                                                    |                                                       aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacc                                              <  1:634129/62‑1 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacc                                              <  1:1762554/62‑1 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacc                                              <  1:2325037/62‑1 (MQ=255) aaaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacc                                              <  1:2884112/62‑1 (MQ=255)  aaaGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTcacca                                             >  1:1133152/1‑62 (MQ=255)        gtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCAcggcgg                                       >  1:628504/1‑62 (MQ=255)        gtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCAcggcgg                                       >  1:3720098/1‑62 (MQ=255)        gtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCAcggcg                                        >  1:1068786/1‑61 (MQ=255)        gtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACAAcggcgg                                       >  1:4444120/1‑62 (MQ=255)        gtgCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCACTGAGGTTTCACCAcggcgg                                       >  1:311802/1‑62 (MQ=255)              gggTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGt                                 <  1:1553818/62‑1 (MQ=255)              gggTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGt                                 <  1:3546603/62‑1 (MQ=255)                  gCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTaaa                              >  1:34196/1‑61 (MQ=255)                  gCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTaaa                              >  1:2455545/1‑61 (MQ=255)                     tttGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCaa                          <  1:1370032/62‑1 (MQ=255)                     tttGCCGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCaa                          <  1:3819756/62‑1 (MQ=255)                         ccGCAGCCTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATAcc                      >  1:4016044/1‑62 (MQ=255)                                cTGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATg               <  1:593410/62‑1 (MQ=255)                                 tGACTCTCCGACGATCCCTGAGGTTTCACCACGGCGGATCt                                   <  1:455927/41‑1 (MQ=38)                                 tGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCt                                   <  1:3889374/41‑1 (MQ=255)                                 tGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCt                                   <  1:306324/41‑1 (MQ=255)                                 tGACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACTATTAATg               <  1:2891764/61‑1 (MQ=255)                                  gACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCt                                   <  1:766548/40‑1 (MQ=255)                                  gACTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGc             <  1:527180/62‑1 (MQ=255)                                   aCTCGCCGACGATCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGc             <  1:4153003/61‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:2049359/62‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:2379072/62‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:2731289/62‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:3358204/62‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:9393/62‑1 (MQ=255)                                             gaTCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGtt  <  1:969507/62‑1 (MQ=255)                                               tCCCTGAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATg               <  1:1672361/47‑1 (MQ=255)                                                    |                                                       AAAAGCTGTGCGAGGGTGCTTTTGCCGCAGCCTGACTCGCCGACGATCCCTAAGGTTTCACCACGGCGGATCTGTAAATCAATACCATTAATGGCATGAACATGTT  >  W3110S.gb/1559568‑1559673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑