Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,584,624	A→G	intergenic (‑386/+16)	ydeN ← / ← ydeO	conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,584,624	0	A	G	100.0%	289.2 / NA	77	intergenic (‑386/+16)	ydeN/ydeO	conserved hypothetical protein/predicted DNA‑binding transcriptional acfivator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/48);  total (29/48)

GCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACC  >  W3110S.gb/1584574‑1584681                                                   |                                                          gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:2661824/62‑1 (MQ=255) gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:4360793/62‑1 (MQ=255) gCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:4328578/62‑1 (MQ=255)    gggAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAaata                                             <  1:480901/62‑1 (MQ=255)    gggAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAaata                                             <  1:4626081/62‑1 (MQ=255)              gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg                                    >  1:4102225/1‑61 (MQ=255)              gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg                                    >  1:4379596/1‑61 (MQ=255)              gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg                                    >  1:1491263/1‑61 (MQ=255)              gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg                                    >  1:1496802/1‑61 (MQ=255)              gCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc                                   <  1:1974726/62‑1 (MQ=255)              gCCCCATTTAATTCTTACGCAGAGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAg                                    >  1:4242304/1‑61 (MQ=255)               ccccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc                                   <  1:273207/61‑1 (MQ=255)                cccATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGc                                   <  1:843245/60‑1 (MQ=255)                  cATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTaa                                >  1:4356778/1‑61 (MQ=255)                  cATTTAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTaa                                >  1:1946039/1‑61 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:3205353/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:3969676/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:3123254/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1091077/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:2965974/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:2108906/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:4291017/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:4304093/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1863191/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1723175/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1687880/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1343280/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1239759/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:119540/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:777203/41‑1 (MQ=255)                      tAATTCTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCAt                           <  1:2602030/62‑1 (MQ=255)                           cTTACGGAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:4199797/36‑1 (MQ=255)                           cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:3485884/36‑1 (MQ=255)                           cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:3048983/36‑1 (MQ=255)                           cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:2562664/36‑1 (MQ=255)                           cTTACGCAGCGTGTGTGGTTGACTGCTCGTTAGCaa                                                <  1:1859306/36‑1 (MQ=255)                           cTTACGCAGCGTGTGTGGTTGACTGCGCGTTAGCaa                                                <  1:3440666/36‑1 (MQ=38)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCa                                         >  1:2997097/1‑41 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:2255960/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:1129668/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:819255/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:520707/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:517845/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:1295167/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:4444678/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:1344317/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:1648356/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:2114825/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:2957530/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:3730189/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:2916960/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:3481922/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:3038542/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:3476344/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:2406376/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgt                    >  1:3460523/1‑62 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg                     <  1:3300265/61‑1 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg                     <  1:3094420/61‑1 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg                     <  1:133763/61‑1 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg                     <  1:4615061/61‑1 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCg                     <  1:2417244/61‑1 (MQ=255)                             tACGCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATCCATCgt                    >  1:237197/1‑62 (MQ=255)                                gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:1888704/60‑1 (MQ=255)                                gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:1168401/60‑1 (MQ=255)                                gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:836920/60‑1 (MQ=255)                                gCAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:3493107/60‑1 (MQ=255)                                 cAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:4575332/59‑1 (MQ=255)                                 cAGCGTGTGTGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCgtg                   <  1:4129560/59‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:2007785/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:3474509/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:3149258/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:2450691/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:306829/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:622272/62‑1 (MQ=255)                                     gtgtgtGGTTGACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCg            <  1:3789974/62‑1 (MQ=255)                                               gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAcc  >  1:1677416/1‑62 (MQ=255)                                               gACTGCTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATAcc  >  1:2209589/1‑62 (MQ=255)                                                   |                                                          GCAGGGAATGGCTGCCCCATTTAATTCTTACGCAGCGTGTGTGGTTGACTACTCGTTAGCAAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACC  >  W3110S.gb/1584574‑1584681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑