Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,607,958	G→A	G49S (GGT→AGT)	lsrF →	predicted aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,607,958	0	G	A	100.0%	76.9 / NA	24	G49S (GGT→AGT)	lsrF	predicted aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/16);  total (8/16)

TTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGG  >  W3110S.gb/1607912‑1608013                                               |                                                        ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:1617401/61‑1 (MQ=255) ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:2405914/61‑1 (MQ=255) ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:2557993/61‑1 (MQ=255) ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:416586/61‑1 (MQ=255) ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:3073246/61‑1 (MQ=255) ttGGGGAATGCAGTCACCCTTATCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATg                                           <  1:1353622/61‑1 (MQ=255)                       tCGCGGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGtt                        >  1:1783405/1‑58 (MQ=255)                         gcgGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTc                  <  1:2565459/62‑1 (MQ=255)                         gcgGATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTc                  <  1:3941635/62‑1 (MQ=255)                           ggATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCa                 >  1:4693767/1‑61 (MQ=255)                           ggATATTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCa                 >  1:461506/1‑61 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATTCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:2965539/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:915298/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:1014130/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:53982/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:3432276/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:1528080/62‑1 (MQ=255)                              tatTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:1173428/62‑1 (MQ=255)                               atTTAATCCGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             <  1:3139066/61‑1 (MQ=255)                                       cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc     >  1:290964/1‑61 (MQ=255)                                       cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc     >  1:4480099/1‑61 (MQ=255)                                       cGAAAACGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTAc     >  1:2517771/1‑61 (MQ=255)                                         aaaaCGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGa             >  1:2328033/1‑51 (MQ=255)                                         aaaaCGAGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCgg  >  1:4575829/1‑62 (MQ=255)                                               |                                                        TTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGG  >  W3110S.gb/1607912‑1608013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑