Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,636,349	G→A	A84V (GCG→GTG)	tfaQ ←	predicted tail fibre assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,636,349	0	G	A	100.0%	49.5 / NA	19	A84V (GCG→GTG)	tfaQ	predicted tail fibre assembly protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/14);  total (5/14)

ATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATG  >  W3110S.gb/1636312‑1636404                                      |                                                        aTTTTCCGGTAACGGACCGAGTTCAGAAATAAGTAACACGTCGCCGGAAGCCACGTCATaaa                                 >  1:3567166/1‑62 (MQ=255) aTTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATaa                                  <  1:2119513/61‑1 (MQ=25)    ttCCGGTAACGGACCGAGTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCg                              >  1:863551/1‑62 (MQ=25)    ttCCGGTAACGGACCGAGTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCg                              >  1:3582635/1‑62 (MQ=25)    ttCCGGTAACGGACCGAGTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCg                              >  1:3337407/1‑62 (MQ=25)    ttCCGGTAACGGACCGAGTTCAGAAATAAAAAACACGTCGcc                                                  >  1:3691409/1‑42 (MQ=14)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:2412450/62‑1 (MQ=25)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:233386/62‑1 (MQ=25)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:3868270/62‑1 (MQ=25)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:3873869/62‑1 (MQ=25)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:3978947/62‑1 (MQ=25)                     gTTCAGAAATAAATAACACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt             <  1:48626/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:2514459/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:3055484/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:3061062/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:1894958/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:3964231/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:4204425/62‑1 (MQ=25)                                aataaCACGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg  <  1:1836401/62‑1 (MQ=25)                                      |                                                        ATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATG  >  W3110S.gb/1636312‑1636404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑