Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,874,182	A→G	Y143C (TAC→TGC)	yeaJ →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,874,182	0	A	G	100.0%	155.4 / NA	42	Y143C (TAC→TGC)	yeaJ	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/30);  total (12/30)

CAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAT  >  W3110S.gb/1874125‑1874231                                                          |                                                  cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg                                                >  1:1643156/1‑61 (MQ=255) cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg                                                >  1:4222728/1‑61 (MQ=255) cagcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCgg                                                >  1:2005842/1‑61 (MQ=255) cagcaCTATTACCCGCTTTTGATCAGGAAGTGGAAGGCAACCGCCACCAGGATGATTGCgg                                                >  1:3713620/1‑61 (MQ=255)   gcaCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGt                                               <  1:1581811/60‑1 (MQ=255)     aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:4535568/62‑1 (MQ=255)     aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:1728966/62‑1 (MQ=255)     aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:179887/62‑1 (MQ=255)     aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:3795596/62‑1 (MQ=255)     aCTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:227339/62‑1 (MQ=255)         ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGTATGATTGCGGTTCAg                                           <  1:2467299/58‑1 (MQ=255)         ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:2547634/58‑1 (MQ=255)         ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:833577/58‑1 (MQ=255)         ttACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAg                                           <  1:1875688/58‑1 (MQ=255)                cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg                                    >  1:862121/1‑58 (MQ=255)                cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg                                    >  1:2468406/1‑58 (MQ=255)                cTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTgg                                    >  1:4553889/1‑58 (MQ=255)                 ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg                               <  1:2975185/62‑1 (MQ=255)                 ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg                               <  1:3461867/62‑1 (MQ=255)                 ttttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg                               <  1:157907/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg                               <  1:3392966/61‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATgg                               <  1:4074376/61‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:1573977/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:1163379/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:3417681/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:1855889/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:3746902/62‑1 (MQ=255)                  tttGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:4636138/62‑1 (MQ=255)                   ttGATCAGGCTGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGc                              <  1:725579/61‑1 (MQ=255)                         aGGCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAaga                        >  1:4596828/1‑61 (MQ=255)                          ggCAGTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAgaga                      >  1:1470646/1‑62 (MQ=255)                              gTGGAAGTCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt                  <  1:2774816/62‑1 (MQ=255)                              gTGGAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt                  <  1:2893773/62‑1 (MQ=255)                                 gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt                  <  1:3691817/59‑1 (MQ=255)                                 gAAGGCAACCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAAtt                  <  1:3346035/59‑1 (MQ=255)                                        aCCGCCACCAGGATGATTGCGGTTCAGGTCTTggg                                   >  1:1260801/1‑35 (MQ=255)                                        aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat           >  1:4583418/1‑59 (MQ=255)                                        aCCGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtat           >  1:1942061/1‑59 (MQ=255)                                          cGCCACCAGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTAtct      <  1:2802776/62‑1 (MQ=255)                                             caccaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGa   <  1:2451811/62‑1 (MQ=255)                                             caccaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGa   <  1:3144743/62‑1 (MQ=255)                                              accaGGATGATTGCGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAt  <  1:412637/62‑1 (MQ=255)                                                          |                                                  CAGCACTATTACCCGCTTTTGATCAGGCAGTGGAAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGAT  >  W3110S.gb/1874125‑1874231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑