Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,002,152	T→C	Y124C (TAC→TGC)	fliY ←	cystine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,002,152	0	T	C	100.0%	49.6 / NA	15	Y124C (TAC→TGC)	fliY	cystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/12);  total (3/12)

CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAG  >  W3110S.gb/2002107‑2002191                                              |                                        ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATc                         <  1:3068132/62‑1 (MQ=255) ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATc                         <  1:3387131/62‑1 (MQ=255) ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATc                         <  1:3850505/62‑1 (MQ=255)   ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCg                        <  1:3608836/61‑1 (MQ=255)     cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTa                      >  1:3580651/1‑61 (MQ=255)     cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTAt                     >  1:720632/1‑62 (MQ=255)      gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCg                        <  1:3249782/58‑1 (MQ=255)                ttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTgcg          >  1:1270567/1‑62 (MQ=255)                ttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTg            <  1:1044821/60‑1 (MQ=255)                ttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTg            <  1:1736895/60‑1 (MQ=255)                ttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTg            <  1:1816107/60‑1 (MQ=255)                ttCACCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTg            <  1:1877129/60‑1 (MQ=255)                   aCCAGCGCCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt        <  1:1949271/61‑1 (MQ=255)                        cgcCTGAATACCAGAAATGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAg  <  1:941065/62‑1 (MQ=255)                                      aaaTGGTGCACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt        <  1:4254841/42‑1 (MQ=255)                                              |                                        CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAG  >  W3110S.gb/2002107‑2002191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑