Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,099,387	T→C	intergenic (+25/+71)	hisI → / ← cld	fused phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,099,387	0	T	C	100.0%	35.9 / NA	11	intergenic (+25/+71)	hisI/cld	fused phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/4);  total (7/4)

CGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTC  >  W3110S.gb/2099367‑2099441                     |                                                       cGGGGTAAGCGGATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTcaccac               >  1:2939322/1‑62 (MQ=255)   gggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaa                                   >  1:1869945/1‑40 (MQ=255)   gggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaa                                   >  1:562067/1‑40 (MQ=255)    ggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaaaa                                 <  1:3087852/41‑1 (MQ=255)    ggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaaaa                                 <  1:3641418/41‑1 (MQ=255)    ggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaaaa                                 <  1:381660/41‑1 (MQ=255)    ggTAAGCGGATGCGATACTGTTGCCGCATCCGGCaaaaaaa                                 <  1:549297/41‑1 (MQ=255)            gATGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCtttt    >  1:1317502/1‑62 (MQ=255)             aTGCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCttttt   >  1:1444246/1‑62 (MQ=255)               gCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCTCCACCCTGCCCTTTTTc  >  1:370456/1‑61 (MQ=255)               gCGATACTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTc  >  1:1616574/1‑61 (MQ=255)                     |                                                       CGGGGTAAGCGGATGCGATATTGTTGCCGCATCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTC  >  W3110S.gb/2099367‑2099441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑