Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,238,432	T→C	L356L (TTA→CTA)	yeiT →	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,238,432	0	T	C	100.0%	82.4 / NA	23	L356L (TTA→CTA)	yeiT	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

AGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCC  >  W3110S.gb/2238377‑2238484                                                        |                                                     aGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGc                                                >  1:698522/1‑62 (MQ=255)  gATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGcc                                               <  1:3876461/62‑1 (MQ=255)    tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGcc                                               <  1:3972866/60‑1 (MQ=255)    tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGcc                                               <  1:2219320/60‑1 (MQ=255)    tAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGcc                                               <  1:3855782/60‑1 (MQ=255)           attCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTaa                                      >  1:2198878/1‑62 (MQ=255)                    gTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCaa                             <  1:4342027/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCTTAACACCATCAAAAcaca                       <  1:449161/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:1346442/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:3873478/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:2362104/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:1933630/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:1501096/62‑1 (MQ=255)                          cAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAAcaca                       <  1:1047372/62‑1 (MQ=255)                              aTGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAAt                   >  1:500137/1‑62 (MQ=255)                                        aGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc         >  1:2018/1‑62 (MQ=255)                                        aGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc         >  1:827342/1‑62 (MQ=255)                                        aGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc         >  1:934027/1‑62 (MQ=255)                                        aGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgaccc         >  1:948608/1‑62 (MQ=255)                                            gCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga     <  1:1666610/62‑1 (MQ=255)                                            gCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcga     <  1:4444224/62‑1 (MQ=255)                                                ttGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgaccc  >  1:2397351/1‑61 (MQ=255)                                                ttGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgaccc  >  1:2179284/1‑61 (MQ=255)                                                        |                                                     AGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCC  >  W3110S.gb/2238377‑2238484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑