Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,358,400	G→A	A362T (GCT→ACT)	glpA →	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,358,400	0	G	A	100.0%	138.0 / NA	42	A362T (GCT→ACT)	glpA	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/35);  total (7/35)

GGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAC  >  W3110S.gb/2358352‑2358458                                                 |                                                           ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:1189464/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:456297/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:647027/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:282834/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:757555/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:248292/62‑1 (MQ=255) ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAc                                               <  1:2366680/62‑1 (MQ=255)  gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:1963660/62‑1 (MQ=255)  gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:1326014/62‑1 (MQ=255)  gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:2862870/62‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:831357/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:2290987/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:1648664/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:146275/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:27175/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:3546114/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:974756/61‑1 (MQ=255)        tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTAATGACTGAATGGGCTACCGAcgc                                         <  1:2866768/61‑1 (MQ=255)           ccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:3978713/53‑1 (MQ=255)                accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:3644510/48‑1 (MQ=255)                accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:1823670/48‑1 (MQ=255)                accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:4671143/48‑1 (MQ=255)                accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:96743/48‑1 (MQ=255)                accGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTAcc                                              <  1:1875152/48‑1 (MQ=255)                       gCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcgc                                         <  1:990599/46‑1 (MQ=255)                         aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:1018153/43‑1 (MQ=255)                         aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:3848561/43‑1 (MQ=255)                         aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:1290296/43‑1 (MQ=255)                         aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:4117312/43‑1 (MQ=255)                         aaaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:1837661/43‑1 (MQ=255)                          aaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:3176548/42‑1 (MQ=255)                          aaCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:2911210/42‑1 (MQ=255)                           aCTGATGACCTATCGGCTGATGACTGAATGGGCTACCGAcg                                          <  1:965191/41‑1 (MQ=255)                            cTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa                                     <  1:3707039/45‑1 (MQ=255)                            cTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa                                     <  1:4120563/45‑1 (MQ=255)                                tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg                >  1:3302929/1‑62 (MQ=255)                                tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg                >  1:532953/1‑62 (MQ=255)                                tgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcg                >  1:1191361/1‑62 (MQ=255)                                         ggCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGt       >  1:3317598/1‑62 (MQ=255)                                              aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc  >  1:4134231/1‑62 (MQ=255)                                              aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc  >  1:2701046/1‑62 (MQ=255)                                              aTGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc  >  1:3758574/1‑62 (MQ=255)                                                 |                                                           GGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAC  >  W3110S.gb/2358352‑2358458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑