Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,493,033 C→T Q1072* (CAG→TAG)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,493,0330CT100.0% 337.0 / NA 101Q1072* (CAG→TAG) evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/56);  total (45/56)

Only 100 of 101 total aligned reads displayed.

GTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGAT  >  W3110S.gb/2492986‑2493080
                                               |                                               
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACAcc                                        >  1:4274860/1‑57 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:4183555/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:2201197/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:211419/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:629422/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:4666343/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:3268067/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:115035/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:3702139/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:3707367/60‑1 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3679524/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3503653/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3292686/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3269835/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3252779/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:315281/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:3901512/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:4069829/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:2992267/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:2914383/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:555451/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:2582645/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:1427428/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:105504/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:2573494/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:1383106/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGtt                                   >  1:1147925/1‑62 (MQ=255)
gtttgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGt                                    >  1:2407928/1‑61 (MQ=255)
gtatgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAg                                     <  1:572255/57‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:4299348/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:1498852/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:3323090/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:3484112/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:3152444/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:1319096/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:604463/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:1087115/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:2813813/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:3827847/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:581654/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:3846399/48‑1 (MQ=255)
   tgttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGt                                              <  1:2453619/48‑1 (MQ=255)
     ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTgcgc                               <  1:4455501/61‑1 (MQ=255)
     ttCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTgcgc                               <  1:4189801/61‑1 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:3845615/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:4036306/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:310422/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:3595912/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:2622686/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:2623995/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:3693286/1‑48 (MQ=255)
          aCCGTTGACCCTGGATGTACTGAAAACACATTTAAGTTAGTTACACCa                                       >  1:4488416/1‑48 (MQ=255)
                      ggATGTACTGAAGACACATTTAAGTTAGTTACACCAAGTTg                                  <  1:4254050/41‑1 (MQ=255)
                      ggATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTg                                  <  1:3888047/41‑1 (MQ=255)
                      ggATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTg                                  <  1:1726831/41‑1 (MQ=255)
                       gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc             >  1:771458/1‑61 (MQ=255)
                       gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc             >  1:2963593/1‑61 (MQ=255)
                       gATGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc             >  1:1732361/1‑61 (MQ=255)
                       gATGTAATGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATc             >  1:1065781/1‑61 (MQ=255)
                         tttACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:709917/60‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:4459254/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:515395/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:48092/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:2853131/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:3644918/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:3639454/62‑1 (MQ=255)
                         tGTACTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGcc          <  1:752550/62‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:4406607/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:4154983/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3604743/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3809163/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:2584932/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3740687/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3718362/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:102002/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3362177/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:2778445/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3082409/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:3047117/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCatat                           >  1:190014/1‑41 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:2071254/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:712485/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:229491/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:2436913/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:2712344/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:4876/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:1127660/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:3352255/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:444526/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:974025/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:3754498/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:4220757/61‑1 (MQ=255)
                             cTGAAAACACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCAcc       <  1:978474/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:2844023/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:1941350/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:621170/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:4065960/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:4638225/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGat  <  1:4262120/61‑1 (MQ=255)
                                  aaCACATTTAAGTTAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGa   <  1:4002287/60‑1 (MQ=255)
                                               |                                               
GTTTGTTCAAACCGTTGACCCTGGATGTACTGAAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGAT  >  W3110S.gb/2492986‑2493080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑