Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	345,099	C→T	A155T (GCC→ACC)	yahN ←	neutral amino‑acid efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	345,099	0	C	T	100.0%	155.9 / NA	47	A155T (GCC→ACC)	yahN	neutral amino‑acid efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/16);  total (31/16)

AATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTA  >  W3110S.gb/345040‑345151                                                            |                                                     aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAt                                                    <  1:2908439/62‑1 (MQ=255)  aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt                                                   <  1:4082574/62‑1 (MQ=255)  aTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTAtt                                                   <  1:256399/62‑1 (MQ=255)             cacaATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         <  1:422023/61‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:403753/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3201538/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:4190591/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:24915/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:4070409/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3583806/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3703460/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3704351/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3782940/52‑1 (MQ=255)               caATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:823937/52‑1 (MQ=255)                aaTCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTaa                                                <  1:3065757/51‑1 (MQ=255)                    cccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAAt                                 >  1:1611622/1‑62 (MQ=255)                    cccGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAAt                                 >  1:3906346/1‑62 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:3584998/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:3308236/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:3255419/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:3234027/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:3213816/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:1027497/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:2979078/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:2726047/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:57338/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:604478/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:2381079/1‑52 (MQ=255)                      cGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACt                                         >  1:2269043/1‑52 (MQ=255)                          cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGa                            >  1:1573600/1‑61 (MQ=255)                                          cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt           >  1:2408057/1‑62 (MQ=255)                                          cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt           >  1:1786855/1‑62 (MQ=255)                                          cccATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt           >  1:753501/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACgg             >  1:4060164/1‑55 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:752549/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:930859/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:347276/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:2976525/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:2519864/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:2410649/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:2090255/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:1566764/1‑62 (MQ=255)                                               gTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCgg      >  1:1293199/1‑62 (MQ=255)                                                  ggTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGt    <  1:1646036/61‑1 (MQ=255)                                                       ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa  >  1:311526/1‑58 (MQ=255)                                                       ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa  >  1:4124965/1‑58 (MQ=255)                                                       ttCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa  >  1:2766904/1‑58 (MQ=255)                                                            |                                                     AATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTA  >  W3110S.gb/345040‑345151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑