Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,792,695	C→T	intergenic (+24/‑214)	gabT → / → gabP	4‑aminobutyrate aminotransferase, PLP‑dependent/gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,792,695	0	C	T	100.0%	69.2 / NA	22	intergenic (+24/‑214)	gabT/gabP	4‑aminobutyrate aminotransferase, PLP‑dependent/gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

TGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAT  >  W3110S.gb/2792656‑2792753                                        |                                                           tgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCt                                       <  1:3403003/61‑1 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTg                                                        >  1:248535/1‑41 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTAc                                     >  1:1503931/1‑60 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACg                                    >  1:2146334/1‑61 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACg                                    >  1:3299898/1‑61 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACg                                    >  1:3542986/1‑61 (MQ=255)    ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACg                                    >  1:569001/1‑61 (MQ=255)         aGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATc                              <  1:1637753/62‑1 (MQ=255)         aGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATc                              <  1:216115/62‑1 (MQ=255)         aGCAGTAGCGCCGCTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATc                              <  1:3311652/62‑1 (MQ=255)                    cgcTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                   <  1:993524/62‑1 (MQ=255)                    cgcTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                   <  1:68781/62‑1 (MQ=255)                    cgcTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                   <  1:4330560/62‑1 (MQ=255)                    cgcTCCTATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                   <  1:3606236/62‑1 (MQ=255)                          tATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCg              >  1:522873/1‑61 (MQ=255)                          tATGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCg              >  1:986485/1‑61 (MQ=255)                           aTGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGtt            >  1:3674709/1‑62 (MQ=255)                           aTGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGtt            >  1:756140/1‑62 (MQ=255)                           aTGCCGGAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGtt            >  1:79180/1‑62 (MQ=255)                                ggAGGCGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgc       <  1:3732984/62‑1 (MQ=255)                                     cGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAt  >  1:3196857/1‑62 (MQ=255)                                     cGATGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAt  >  1:2465806/1‑62 (MQ=255)                                        |                                                           TGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAT  >  W3110S.gb/2792656‑2792753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑