Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,863,059	G→A	L56L (CTG→CTA)	ygbM →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,863,059	0	G	A	100.0%	164.2 / NA	49	L56L (CTG→CTA)	ygbM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/33);  total (16/33)

CCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGC  >  W3110S.gb/2863000‑2863115                                                            |                                                         cccTATAACTACTCCCCCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        >  1:4187244/1‑62 (MQ=255) cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        >  1:563871/1‑62 (MQ=255) cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        >  1:3822097/1‑62 (MQ=255) cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        >  1:389109/1‑62 (MQ=255)  ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:2653365/61‑1 (MQ=255)  ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:3814772/61‑1 (MQ=255)  ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:206852/61‑1 (MQ=255)                 cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt                                                     <  1:2156656/49‑1 (MQ=255)                 cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt                                                     <  1:1923090/49‑1 (MQ=255)                  ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt                                                     <  1:3298220/48‑1 (MQ=255)                  ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc                                       <  1:4074004/62‑1 (MQ=255)                  ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc                                       <  1:1854332/62‑1 (MQ=255)                  ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc                                       <  1:496846/62‑1 (MQ=255)                  ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc                                       <  1:1108515/62‑1 (MQ=255)                      cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:90172/41‑1 (MQ=255)                      cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:4203686/41‑1 (MQ=255)                      cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc                                                        <  1:437060/41‑1 (MQ=255)                        aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt                                              >  1:1594540/1‑49 (MQ=255)                        aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt                                              >  1:280248/1‑49 (MQ=255)                        aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt                                              >  1:2213292/1‑49 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGgaga                                >  1:2717828/1‑61 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat                               >  1:730616/1‑62 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat                               >  1:663295/1‑62 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat                               >  1:65407/1‑62 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat                               >  1:249539/1‑62 (MQ=255)                          tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGAGCTGTTCAATACCGCTCCCGgag                                 >  1:3592594/1‑60 (MQ=255)                           ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg                                   <  1:3619619/57‑1 (MQ=255)                           ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg                                   <  1:1789694/57‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:2621501/62‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:1715176/62‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:2006752/62‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:3601358/62‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:3435843/62‑1 (MQ=255)                                aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:3365179/62‑1 (MQ=255)                                 gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg                        >  1:3639684/1‑62 (MQ=255)                                 gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg                        >  1:751154/1‑62 (MQ=255)                                 gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg                        >  1:745793/1‑62 (MQ=255)                                    aCTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg                                   <  1:76494/48‑1 (MQ=255)                                         aGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt                         <  1:893987/53‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGTAATg              <  1:1729151/62‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:1006300/62‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:768517/62‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:2743349/62‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:3983318/62‑1 (MQ=255)                                           cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:2848272/62‑1 (MQ=255)                                            aaaaCCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:3184101/61‑1 (MQ=255)                                             aaaCCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg              <  1:1323958/60‑1 (MQ=255)                                                         acTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGc  <  1:4549110/60‑1 (MQ=255)                                                         acTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGc  <  1:1922567/60‑1 (MQ=255)                                                            |                                                         CCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGC  >  W3110S.gb/2863000‑2863115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑