Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,966,215	T→G	K292N (AAA→AAC)	ptsP ←	fused PEP‑protein phosphotransferase (enzyme I) of PTS system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,966,215	0	T	G	100.0%	123.1 / NA	34	K292N (AAA→AAC)	ptsP	fused PEP‑protein phosphotransferase (enzyme I) of PTS system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/20);  total (14/20)

CAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACC  >  W3110S.gb/2966163‑2966269                                                     |                                                       caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATa                                                >  1:1234875/1‑61 (MQ=255) caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATa                                                >  1:1616846/1‑61 (MQ=255) caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATa                                                >  1:3616726/1‑61 (MQ=255)      tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc                                          >  1:678521/1‑62 (MQ=255)      tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc                                          >  1:293212/1‑62 (MQ=255)      tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc                                          >  1:2349060/1‑62 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:877373/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:1173082/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:4122498/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:2471439/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:2237761/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:2279464/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:1451117/62‑1 (MQ=255)                 tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCCCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:2696863/62‑1 (MQ=255)                 tCGCTGACCCAGCCCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:303801/62‑1 (MQ=255)                          cAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc                                          >  1:1245936/1‑42 (MQ=255)                          cAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc                                          >  1:2541888/1‑42 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:2690666/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:330499/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:642544/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:1677439/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:2597269/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:3844516/62‑1 (MQ=255)                                aCGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa                <  1:4015697/62‑1 (MQ=255)                                     aaTCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCgg                               <  1:252367/42‑1 (MQ=255)                                           cAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt      <  1:365118/61‑1 (MQ=255)                                           cAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt      <  1:705370/61‑1 (MQ=255)                                           cAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt      <  1:1421660/61‑1 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCaa                            >  1:253557/1‑36 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtt             >  1:3342484/1‑51 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGAcc  >  1:3602086/1‑62 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGAcc  >  1:648155/1‑62 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGAcc  >  1:1930784/1‑62 (MQ=255)                                              cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGAcc  >  1:2509725/1‑62 (MQ=255)                                                     |                                                       CAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACC  >  W3110S.gb/2966163‑2966269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑