Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,001,511	T→C	I81T (ATA→ACA)	xdhB →	xanthine dehydrogenase, FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,001,511	0	T	C	100.0%	230.2 / NA	61	I81T (ATA→ACA)	xdhB	xanthine dehydrogenase, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/23);  total (38/23)

CTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGG  >  W3110S.gb/3001456‑3001558                                                        |                                                cTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACagaaga                                           >  1:3951857/1‑62 (MQ=255) cTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACagaaga                                           >  1:3379735/1‑62 (MQ=255)   ggCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGAt                                          >  1:2467200/1‑61 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:174203/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:3816887/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:2585585/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:4126961/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:3738345/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:4173656/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:1762047/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:3702772/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:320275/61‑1 (MQ=255)      ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCt                                       <  1:3082281/61‑1 (MQ=255)                        tATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCAtctc                     <  1:541547/61‑1 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCgg                 >  1:4415220/1‑61 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:2983259/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:3098500/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:2907555/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:2940486/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:4161578/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:1752863/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:4555090/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:4556093/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:1480957/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:4564201/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:142039/1‑62 (MQ=255)                            ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:1374033/1‑62 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCtat                                     >  1:679488/1‑40 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:1300153/1‑61 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:1697147/1‑61 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:302766/1‑61 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:4242734/1‑61 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:3510286/1‑61 (MQ=255)                             gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc                >  1:2639202/1‑61 (MQ=255)                               tctGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt             <  1:3431593/62‑1 (MQ=255)                               tctGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt             <  1:368669/62‑1 (MQ=255)                                ctGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt             <  1:3771675/61‑1 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:1544681/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:1496220/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:1976204/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:1449240/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:2625731/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:4668170/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt              >  1:1396094/1‑57 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:2632737/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:4664990/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:2969548/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:1834137/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:2867799/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:4225151/1‑59 (MQ=255)                                   cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa            >  1:2963496/1‑59 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:536055/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:2129713/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:4223763/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:4153498/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:248408/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:3000010/62‑1 (MQ=255)                                          aTTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:2995794/62‑1 (MQ=255)                                           tttACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:4516594/61‑1 (MQ=255)                                                 cAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  >  1:2754001/1‑55 (MQ=255)                                                     tAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg  <  1:2853536/51‑1 (MQ=255)                                                        |                                                CTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGG  >  W3110S.gb/3001456‑3001558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑