Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,139,938	C→T	R2H (CGT→CAT)	hybD ←	predicted maturation element for hydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,139,938	0	C	T	100.0%	175.7 / NA	53	R2H (CGT→CAT)	hybD	predicted maturation element for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

CGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAC  >  W3110S.gb/3139881‑3139995                                                          |                                                          cGCATACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:2929274/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:3674616/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:3784926/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:4022122/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:671916/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:1307808/61‑1 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa                                                        <  1:577942/61‑1 (MQ=255)     cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACa                                                   <  1:4342164/62‑1 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGCCCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:1113057/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAg                                                  >  1:1403655/1‑60 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:3172641/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:2824707/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:2483905/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:239083/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:1690677/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:1693159/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:1199790/1‑61 (MQ=255)        cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa                                                 >  1:4215555/1‑61 (MQ=255)           tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt                                             <  1:4052877/62‑1 (MQ=255)           tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt                                             <  1:3004769/62‑1 (MQ=255)           tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt                                             <  1:4514728/62‑1 (MQ=255)            ggCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt                                            <  1:4669058/62‑1 (MQ=255)             gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt                                            <  1:3748300/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:4219678/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:1011420/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:3454957/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:1537514/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:2991506/61‑1 (MQ=255)               ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa                                          <  1:1462523/61‑1 (MQ=255)                       tCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCa                                 <  1:1597969/62‑1 (MQ=255)                              aaTATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt                                            <  1:1868371/44‑1 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:895207/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:449542/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:947414/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:2386784/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:1956511/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:1602369/1‑62 (MQ=255)                               atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg                         >  1:1310850/1‑62 (MQ=255)                                             tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc                    <  1:2832558/53‑1 (MQ=255)                                             tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc                    <  1:2904665/53‑1 (MQ=255)                                             tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc                    <  1:1864490/53‑1 (MQ=255)                                             tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc                    <  1:1682327/53‑1 (MQ=255)                                                     aaaTATGCATTACAGAACCTTCACTGAACCCACTTCGTTGCCGTCAGCATCCACTACgtgt    >  1:2452854/1‑61 (MQ=255)                                                     aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt    >  1:1327307/1‑61 (MQ=255)                                                     aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa   >  1:1133907/1‑62 (MQ=255)                                                     aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa   >  1:127999/1‑62 (MQ=255)                                                     aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa   >  1:1994603/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:18920/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:1201302/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:584994/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:2290508/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:246963/1‑62 (MQ=255)                                                      aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc  >  1:3342631/1‑62 (MQ=255)                                                          |                                                          CGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAC  >  W3110S.gb/3139881‑3139995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑