Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,191,865	G→A	A116T (GCC→ACC)	yqiK →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,191,865	0	G	A	100.0%	83.0 / NA	27	A116T (GCC→ACC)	yqiK	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

TGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGT  >  W3110S.gb/3191807‑3191917                                                           |                                                     tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:4382649/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:4366816/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:1737004/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:4095550/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:4090189/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:2526248/61‑1 (MQ=255) tGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTAcc                                                    <  1:3420686/61‑1 (MQ=255)      tagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              >  1:2300744/1‑62 (MQ=255)      tagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCAACCGCTACCCa                                                  >  1:3441244/1‑58 (MQ=255)       agtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              <  1:1021842/61‑1 (MQ=255)       agtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              <  1:4204744/61‑1 (MQ=255)       agtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              <  1:4086229/61‑1 (MQ=255)       agtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              <  1:276015/61‑1 (MQ=255)       agtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACg                                              <  1:1720733/61‑1 (MQ=255)        gtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCt                                            >  1:2214971/1‑62 (MQ=255)           gCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGc                                             <  1:741995/58‑1 (MQ=255)           gCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGc                                             <  1:1242081/58‑1 (MQ=255)                  ttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGc                                  >  1:642670/1‑62 (MQ=255)                  ttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGc                                  >  1:516614/1‑62 (MQ=255)                      gCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCAccc                              >  1:893196/1‑62 (MQ=255)                                    cAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGa                 >  1:2157526/1‑61 (MQ=255)                                               aTTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt     >  1:4417460/1‑62 (MQ=255)                                               aTTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt     >  1:4413542/1‑62 (MQ=255)                                               aTTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt     >  1:3979988/1‑62 (MQ=255)                                                      ccGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggt  >  1:4503970/1‑58 (MQ=255)                                                      ccGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggt  >  1:599183/1‑58 (MQ=255)                                                      ccGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggt  >  1:1105370/1‑58 (MQ=255)                                                           |                                                     TGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGT  >  W3110S.gb/3191807‑3191917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑