Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	926,386	Δ2 bp	noncoding (7‑8/88 nt)	serW ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	926,384	0	A	.	100.0%	63.2 / NA	17	noncoding (10/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (0/17);  total (0/17)
*	W3110S.gb	926,385	0	C	.	100.0%	63.2 / NA	17	noncoding (9/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (0/17);  total (0/17)

CCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGG  >  W3110S.gb/926369‑926431                ||                                               ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:577280/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:808787/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:779579/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:685370/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:676852/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:639772/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:618663/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:591109/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:58224/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:1031386/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:525059/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:417151/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:155536/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:138220/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:1337456/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:116911/61‑1 (MQ=38) ccTTCAGCCACTCGG‑‑ACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACggg  <  1:1099307/61‑1 (MQ=38)                ||                                               CCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGG  >  W3110S.gb/926369‑926431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑