Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,504,241	Δ1 bp	coding (125/1698 nt)	ypdA →	predicted sensory kinase in two‑component system with YpdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,504,238	0	C	.	100.0%	32.3 / NA	9	coding (122/1698 nt)	ypdA	predicted sensory kinase in two‑component system with YpdB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/0);  total (9/0)

GTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCAT  >  W3110S.gb/2504206‑2504268                                 |                               gTTTCGCGAACTGTTGCACAAGTCGTCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:722547/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCa   >  1:1178450/1‑61 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCa   >  1:575415/1‑61 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:380600/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:420767/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:426802/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:6160/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:989152/1‑62 (MQ=255) gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt  >  1:993772/1‑62 (MQ=255)                                 |                               GTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCAT  >  W3110S.gb/2504206‑2504268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑