Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,789,435	Δ1 bp	coding (111/291 nt)	ilvN →	acetolactate synthase I, small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,789,434	0	A	.	100.0%	80.0 / NA	20	coding (110/291 nt)	ilvN	acetolactate synthase I, small subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/0);  total (20/0)

ACGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTC  >  W3110S.gb/3789395‑3789457                                        |                        aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGAtt   >  1:194544/1‑61 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:1058825/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:963538/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:93564/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:930480/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:824862/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:78118/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:734889/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:630571/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:589040/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:567950/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:197803/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:194123/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:192571/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:137393/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:1372508/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:1359670/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:1301999/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:1056031/1‑62 (MQ=255) aCGTTTGTGGCCTTTTTGCCC‑CCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc  >  1:414517/1‑61 (MQ=255)                                        |                        ACGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTC  >  W3110S.gb/3789395‑3789457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑