Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	88,730	+A	coding (703/1005 nt)	fruR →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	88,727	1	.	A	100.0%	103.3 / NA	39	E234K (GAA→AAA)	fruR	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/39);  total (0/39)

AGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGG‑AAACGCATCCGATGCCGCAGG  >  W3110S.gb/88689‑88748                                        |                      aGCGGGAGGCGGCTGCCTAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:61144/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAGAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1333626/61‑1 (MQ=38) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:750811/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:467619/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:557558/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:564536/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:686480/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:695855/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:70679/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:709047/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:747930/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:391736/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:764863/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:83050/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:861398/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:873188/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:896110/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:898208/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:902475/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1431363/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1123115/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1140083/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1209317/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1217787/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1224403/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:128308/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1367366/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1386542/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1406388/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1079929/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1440907/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:144259/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:1462081/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:208004/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:312687/61‑1 (MQ=255) aGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:316077/61‑1 (MQ=255) aGCGGGAGGCGGCAGCCCAGTTATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:702289/61‑1 (MQ=255)                      tATTCGAAAAATGGCTGGAAAACGCATCCGATGCCGCAgg  <  1:504802/40‑1 (MQ=38)                         tCGAAAAATGGCTGGAAAACGCATCCGATGCCGAAgg  <  1:206300/37‑1 (MQ=25)                                        |                      AGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGG‑AAACGCATCCGATGCCGCAGG  >  W3110S.gb/88689‑88748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑