Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	771,134	+A	intergenic (+101/‑746)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	771,130	1	.	A	100.0%	36.7 / NA	16	intergenic (+97/‑750)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/16);  total (0/16)

GATACGGCATGAACGCTTTATTCGGTCTAC‑AAAAGCAGGCAAATTCAATATATTGCAGAGA  >  W3110S.gb/771101‑771161                               |                                gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:309307/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:385649/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:407746/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:45713/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:489013/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:533994/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:542671/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:555666/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:562258/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:744197/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:914326/62‑1 (MQ=255) gATACGGCATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:987993/62‑1 (MQ=255) gATACGACATGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:1027554/62‑1 (MQ=21)          tGAACGCTTTATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:82578/53‑1 (MQ=18)                 tttATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:1102173/46‑1 (MQ=18)                 tttATTCGGTCTACAAAAAGCAGGCAAATTCAATATATTGCAgaga  <  1:557840/46‑1 (MQ=18)                               |                                GATACGGCATGAACGCTTTATTCGGTCTAC‑AAAAGCAGGCAAATTCAATATATTGCAGAGA  >  W3110S.gb/771101‑771161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑