Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,454,872	+C	coding (485/2046 nt)	maoC ←	fused aldehyde dehydrogenase and enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,454,868	1	.	C	100.0%	27.9 / NA	12	G163G (GGA→GGG)	maoC	fused aldehyde dehydrogenase and enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (12/0);  total (12/0)

GCTTTTCCAGCATT‑CCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGA  >  W3110S.gb/1454855‑1454915               |                                                gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:1146905/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:1188449/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:1196833/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:1357690/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:193116/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:322965/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:465543/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:718612/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:730499/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:739599/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:785355/1‑62 (MQ=255) gCTTTTCCAGCATTCCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGa  >  1:788774/1‑62 (MQ=255)               |                                                GCTTTTCCAGCATT‑CCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCACTGCCACGCCTGA  >  W3110S.gb/1454855‑1454915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑