Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,509,623	+A	coding (1129/1962 nt)	ydcP →	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,509,620	1	.	A	100.0%	44.8 / NA	18	E376K (GAA→AAA)	ydcP	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/18);  total (0/18)

CAATG‑AAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTT  >  W3110S.gb/1509616‑1509676      |                                                         cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:521825/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:981050/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:924936/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:831511/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:82649/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:721101/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:673812/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:592458/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:582258/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:1035200/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:368290/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:258812/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:209634/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:138487/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:128739/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:114584/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:1135526/62‑1 (MQ=255) cAATGAAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCtt  <  1:1122203/62‑1 (MQ=255)      |                                                         CAATG‑AAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTT  >  W3110S.gb/1509616‑1509676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑