Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,024,514	+T	coding (509/738 nt)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,024,510	1	.	T	100.0%	63.5 / NA	25	I169F (ATT→TTT)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/0);  total (25/0)

GTTGCAGGGACCTGAAGCCGTGCCGATGCGCA‑TTTTGCTCCCGGCCTACGTGACCAGCGAG  >  W3110S.gb/2024479‑2024539                                 |                              gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCGGGCCTACGTGACCAGCGAg  >  1:900470/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGc                  >  1:816704/1‑46 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGCCCAGCGAg  >  1:1389364/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGa   >  1:307171/1‑61 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:258595/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:933873/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:791938/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:709387/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:662892/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:607468/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:390597/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:260779/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:258725/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1017817/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:25603/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:244387/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1446706/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1430810/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:141858/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1417386/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1250723/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:124241/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1179513/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCCTACGTGACCAGCGAg  >  1:1054926/1‑62 (MQ=255) gTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTTGCTCCCGGCATACGTGACCAGCGa   >  1:511872/1‑61 (MQ=255)                                 |                              GTTGCAGGGACCTGAAGCCGTGCCGATGCGCA‑TTTTGCTCCCGGCCTACGTGACCAGCGAG  >  W3110S.gb/2024479‑2024539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑