Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,076,801 +TG intergenic (+6/‑115) flu → / → yeeR antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/predicted membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,076,8011.T100.0% 79.6 / NA 33intergenic (+6/‑115)flu/yeeRantigen 43 (Ag43) phase‑variable biofilm formation autotransporter/predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (33/0);  total (33/0)
*W3110S.gb2,076,8012.G100.0% 82.1 / NA 33intergenic (+6/‑115)flu/yeeRantigen 43 (Ag43) phase‑variable biofilm formation autotransporter/predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (33/0);  total (33/0)

ACTGAATGTGACCTTCTGACAGAAC‑‑CATCGCCTCTCTGTGGTCCCGGTCATCATGACCGG  >  W3110S.gb/2076777‑2076836
                         ||                                   
tcTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:940647/2‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGt                    >  1:936904/1‑44 (MQ=9)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGt                    >  1:395821/1‑44 (MQ=9)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGt                    >  1:341524/1‑44 (MQ=9)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGt                    >  1:1007458/1‑44 (MQ=9)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGt                    >  1:1369532/1‑44 (MQ=9)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTccccg               >  1:1202520/1‑47 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTccc                 >  1:181413/1‑47 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTccc                 >  1:68150/1‑47 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTccc                 >  1:313170/1‑47 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTccc                 >  1:1085473/1‑47 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1212501/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:81100/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:733099/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:726139/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:680674/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:458300/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1103462/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:395590/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1143025/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:275382/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:235639/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1227420/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:176996/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:156520/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1440198/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1392961/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTcc                  >  1:1264332/1‑46 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGt              >  1:1016751/1‑50 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGt              >  1:1227851/1‑50 (MQ=255)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCgg  >  1:794475/1‑62 (MQ=19)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCgg  >  1:81725/1‑62 (MQ=19)
acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGt              >  1:108352/1‑50 (MQ=255)
                         ||                                   
ACTGAATGTGACCTTCTGACAGAAC‑‑CATCGCCTCTCTGTGGTCCCGGTCATCATGACCGG  >  W3110S.gb/2076777‑2076836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: