| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 232,620 | (GTT)3→4 | coding (1336/1359 nt) | mltD ← | predicted membrane‑bound lytic murein transglycosylase D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 232,610 | 1 | . | T | 100.0% | 92.5 / NA | 35 | M449K (ATG→AAG) | mltD | predicted membrane‑bound lytic murein transglycosylase D |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (35/0); total (35/0) | |||||||||||
| * | W3110S.gb | 232,610 | 2 | . | G | 100.0% | 96.2 / NA | 35 | M449T (ATG→ACG) | mltD | predicted membrane‑bound lytic murein transglycosylase D |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (35/0); total (35/0) | |||||||||||
| * | W3110S.gb | 232,610 | 3 | . | T | 100.0% | 92.7 / NA | 35 | M449K (ATG→AAG) | mltD | predicted membrane‑bound lytic murein transglycosylase D |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (35/0); total (35/0) | |||||||||||
TATTATCTGGTTTGTCAGGAATCTGGCA‑‑‑TGTTGTTGTTTTTCACAAACAACGTCAGCTT > W3110S.gb/232583‑232641 ||| tattatCTGGTTTGTCAGGTATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:256427/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGc > 1:1140709/1‑60 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGc > 1:477369/1‑60 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:667247/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:396670/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:405498/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:521752/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:533926/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:566638/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:604498/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:350090/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:682064/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:698673/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:712436/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:795885/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:928121/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:974631/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1263308/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1032105/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1116756/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1150251/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1184902/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1212289/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1232950/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1010108/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1275800/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1278990/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1294372/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1341282/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:1350211/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:262409/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCtt > 1:346953/1‑62 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCt > 1:666809/1‑61 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCt > 1:743352/1‑61 (MQ=255)tattatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTGTTTTTCACAAACAACGTCAGCt > 1:790203/1‑61 (MQ=255) ||| TATTATCTGGTTTGTCAGGAATCTGGCA‑‑‑TGTTGTTGTTTTTCACAAACAACGTCAGCTT > W3110S.gb/232583‑232641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |