Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,661,826	+C	coding (154/741 nt)	yfhQ ←	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,661,822	1	.	C	100.0%	157.8 / NA	56	A53G (GCC→GGC)	yfhQ	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (0/56);  total (0/56)

TACCAATCACATCGCTGG‑CCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAG  >  W3110S.gb/2661805‑2661865                   |                                            tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTTGGAGTCGGGTTTCACCAg  <  1:1317614/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:604418/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1034654/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:363048/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:370660/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:446516/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:483650/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:489198/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:498561/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:545255/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:567380/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:573598/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:575454/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:580899/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:590561/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:331125/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:654955/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:673286/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:675411/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:790489/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:793674/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:813292/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:838382/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:850479/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:8837/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:937271/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:957285/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:958343/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1388783/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1113922/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1148278/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1180017/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1207721/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:122559/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1265989/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1270601/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1306061/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1308366/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1343505/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1043239/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1415626/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1438839/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1470273/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:159741/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:163528/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:183801/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:187784/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:189629/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:200283/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:228379/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:251267/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:306216/62‑1 (MQ=255) tACCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGCGAGTCGGGTTTCACCAg  <  1:701793/62‑1 (MQ=255)  aCCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:334797/61‑1 (MQ=255)  aCCAATCACATCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1063217/61‑1 (MQ=255)         acaTCGCTGGCCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg  <  1:1354215/54‑1 (MQ=39)                   |                                            TACCAATCACATCGCTGG‑CCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAG  >  W3110S.gb/2661805‑2661865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑