| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,874,423 | +GT | coding (563/909 nt) | cysD ← | sulfate adenylyltransferase, subunit 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,874,421 | 1 | . | G | 100.0% | 34.6 / NA | 14 | W189R (TGG→CGG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/0); total (14/0) | |||||||||||
| * | W3110S.gb | 2,874,421 | 2 | . | T | 100.0% | 32.9 / NA | 14 | W189R (TGG→AGG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (14/0); total (14/0) | |||||||||||
ATATCCTGCTCGGTCCA‑‑GTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATT > W3110S.gb/2874405‑2874464 || atatCCTGCTCTGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:351630/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaaat > 1:300028/1‑60 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:1035726/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:1045908/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:124011/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:152732/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:497384/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:589184/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:691994/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:913074/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:956110/1‑62 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:1256437/1‑61 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:181329/1‑61 (MQ=255)atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:675393/1‑61 (MQ=255) || ATATCCTGCTCGGTCCA‑‑GTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATT > W3110S.gb/2874405‑2874464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |