Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,292,336	+C	coding (855/861 nt)	yraL ←	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,292,332	1	.	C	77.8%	37.2 / 7.5	18	coding (859/861 nt)	yraL	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (4/0);  new base C (0/14);  total (4/14)
Fisher's exact test for biased strand distribution p-value = 3.27e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCCTGCTGCTCCAGC  >  W3110S.gb/3292277‑3292348                                                         |                 tCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCctt            >  1:659757/1‑61 (MQ=255) tCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCctg            >  1:134644/1‑62 (MQ=255) tCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCctg            >  1:228877/1‑62 (MQ=255) tCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCctg            >  1:641235/1‑62 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:629434/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:990167/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:925221/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:896332/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:889609/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:687703/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:6567/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:102594/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:59098/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:424959/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:290098/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:21446/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:1391735/51‑1 (MQ=255)                       gCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCCTGCTGCTCCAgc  <  1:1057692/51‑1 (MQ=255)                                                         |                 TCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTA‑CCCCTGCTGCTCCAGC  >  W3110S.gb/3292277‑3292348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑