| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,429,766 | +A | coding (618/1590 nt) | purH → | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,429,763 | 1 | . | A | 100.0% | 18.7 / NA | 9 | S205R (AGC→AGA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/9); total (0/9) | |||||||||||
GCATGGTTCCGGCTTACCACGGTGAAAGC‑AAAGAAGCCGCCGGTCGCTTCCCACGCACGCT > W3110S.gb/3429735‑3429795 | gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1013963/62‑1 (MQ=255)gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1425907/62‑1 (MQ=255)gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1440393/62‑1 (MQ=255)gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:25206/62‑1 (MQ=255)gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:858460/62‑1 (MQ=255)gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:967629/62‑1 (MQ=255) caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1212924/61‑1 (MQ=255) caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1363040/61‑1 (MQ=255) caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:429546/61‑1 (MQ=255) | GCATGGTTCCGGCTTACCACGGTGAAAGC‑AAAGAAGCCGCCGGTCGCTTCCCACGCACGCT > W3110S.gb/3429735‑3429795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |