Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,891,157	(T)5→6	coding (29/861 nt)	sgbU ←	predicted L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,891,152	1	.	T	100.0%	106.7 / NA	40	A12T (GCG→ACG)	sgbU	predicted L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (40/0);  total (40/0)

TTCGCCAGCGC‑TTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATA  >  W3110S.gb/3891142‑3891202            |                                                   ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCAcgcg                >  1:318358/1‑48 (MQ=39) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAg     >  1:1182275/1‑59 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGAt   >  1:448578/1‑61 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:151508/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:150160/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:21753/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:291539/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:376812/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:407423/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:415888/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:429144/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:461877/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:655734/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:716329/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:732442/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:778202/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:796364/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:845243/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:891006/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:900745/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1142662/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1024048/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1029122/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1031337/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1061030/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1093440/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1099109/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1108260/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1123671/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1013901/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1160961/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1183888/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1196184/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1224975/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1265869/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1301154/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1439944/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1443362/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCCTAACTGATGATTACGAACGCGCACCTCCCCAGATa  >  1:537330/1‑62 (MQ=255) ttCGCCAGCGCTTTTTTCATAAATCCATAACTGATGATTACGCACGCGCACCTCCCCAGATa  >  1:1250771/1‑62 (MQ=255)            |                                                   TTCGCCAGCGC‑TTTTTCATAAATCCCTAACTGATGATTACGCACGCGCACCTCCCCAGATA  >  W3110S.gb/3891142‑3891202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑