Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	2541187	2541260	74	38 [0]	[0] 4	[ptsI]	[ptsI]

TAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGG  >  W3110S.gb/2541125‑2541186                                                              | tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:670722/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:355773/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:503199/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:50933/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:512908/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:513551/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:587414/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:639008/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:641821/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1024679/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:726039/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:817402/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:839973/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:856546/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:899596/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:914134/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:92229/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:969427/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1347094/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:109311/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1096448/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:107551/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1153558/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1191864/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1238463/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1067553/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1279245/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1407759/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:182353/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:202672/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:298970/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:316815/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1014479/62‑1 (MQ=255) tAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:341236/62‑1 (MQ=255)  aaCACGAACTTCGAAGATGCGAAGGTTTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:403821/61‑1 (MQ=255)  aaCACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:666851/61‑1 (MQ=255)   acacGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:497487/60‑1 (MQ=255)                         ggTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACgg  <  1:1125614/38‑1 (MQ=255)                                                              | TAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGG  >  W3110S.gb/2541125‑2541186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑