Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4257543	4257651	109	11 [0]	[0] 44	[plsB]	[plsB]

GATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTC  >  W3110S.gb/4257482‑4257542                                                             | gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:1280330/61‑1 (MQ=255) gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:1470857/61‑1 (MQ=255) gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:243236/61‑1 (MQ=255) gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:306385/61‑1 (MQ=255) gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:762561/61‑1 (MQ=255) gatgTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:858278/61‑1 (MQ=255)                    atcatcCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:698398/42‑1 (MQ=255)                       atcCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:559875/39‑1 (MQ=255)                         ccGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:257444/37‑1 (MQ=255)                         ccGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:591287/37‑1 (MQ=255)                          cGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTtc  <  1:178213/36‑1 (MQ=255)                                                             | GATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTC  >  W3110S.gb/4257482‑4257542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑