Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4590448	4590574	127	8 [0]	[0] 57	hsdR	endonuclease R

GCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACA  >  W3110S.gb/4590387‑4590447                                                             | gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGACGCCATTATCGAAACa  >  1:104370/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:1264485/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:1278428/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:1365862/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:470164/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:814566/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACa  >  1:864085/1‑61 (MQ=255) gCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGATTCCCGCAGGAAGCCATTATCGAAACa  >  1:906181/1‑61 (MQ=255)                                                             | GCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACA  >  W3110S.gb/4590387‑4590447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑