Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	131917	131926	10	30 [0]	[0] 47	acnB	bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase

CTGACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATCC  >  W3110S.gb/131855‑131916                                                              | ctgACTCCGGAGAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:552366/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:265370/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:972172/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:936784/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:908016/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:889126/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:883034/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:769446/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:576974/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:554562/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:53265/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:5300/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:498896/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:346685/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1000583/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:260697/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:245381/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:180281/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:17528/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:159587/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1413950/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1356058/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1334842/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1294538/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1293511/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1205856/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:111095/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATcc  >  1:1008007/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCACGGTGGTTACAACATTCATcc  >  1:897751/1‑62 (MQ=255) ctgACTCCGGAAAAAGCCATCGAACTGCTGGGCACAATGCAGGGTGGTTACAACATTCATcc  >  1:1006392/1‑62 (MQ=255)                                                              | CTGACTCCGGAAAAAGCCATCGAACTGCTGGGCACCATGCAGGGTGGTTACAACATTCATCC  >  W3110S.gb/131855‑131916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑