Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	3685420	3685459	40	43 [0]	[0] 22	ilvG	Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II

CAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAC  >  W3110S.gb/3685460‑3685521 |                                                              cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTgcgc                           >  1:1087718/1‑37 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCCCCGCCAACGTAc  >  1:851520/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTa   >  1:168298/1‑61 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTa   >  1:375248/1‑61 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:458789/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:866583/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:831903/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:8316/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:679086/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:634031/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:549975/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:527693/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:494238/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:463677/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:1020859/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:380456/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:28704/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:1268472/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:1079489/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:1044014/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAc  >  1:1031583/1‑62 (MQ=255) cAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCACCAACGTAc  >  1:1135548/1‑62 (MQ=255) |                                                              CAGCGAGAAATTCACGCAAAGCCGGAACTGCCTGCGCCATACCCACGCCACCGCCAACGTAC  >  W3110S.gb/3685460‑3685521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑