| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,142,832 | 0 | T | C | 74.6% | 164.6 / 51.1 | 118 | E306E (GAA→GAG) | asmA | predicted assembly protein |
| Reads supporting (aligned to +/- strand): ref base T (25/5); new base C (88/0); total (113/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 118 total aligned reads displayed.
GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG > W3110S.gb/2142786‑2142864 | ggCGCGGCATCGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1068901/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCTGCACCGTTCTCACCGTTCGCTGt > 1:241975/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCAccg > 1:1118140/1‑52 (MQ=39)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:387688/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:691118/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:764676/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:1253271/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:1070711/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:522655/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:92507/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgct > 1:1091515/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgc > 1:1052412/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:265200/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:381014/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:326377/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:79568/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:293364/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:735802/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:252526/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:249323/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:800887/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:166484/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1470036/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:40128/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:429365/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:754312/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:454994/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1414812/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:729820/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:488515/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:510954/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:518248/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:52945/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:729353/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:590564/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:705267/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:618871/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:636359/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:693340/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:981995/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1467305/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:959890/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1134899/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1119112/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1101798/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1100553/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1094153/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1092050/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1157887/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1058118/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1051396/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:104485/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1029225/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1015412/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1015116/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:985917/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1308625/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:852649/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1408605/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:898924/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1378298/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:908991/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:956311/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1291713/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:909452/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1233993/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1233901/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:931188/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1201575/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTAACCGTTCGCTGt > 1:374771/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCAGCCCTTGCTGCGCGGCACCGTTCTCACCGTTc > 1:683260/1‑56 (MQ=255)ggCGCGGCAACGTTGATTAGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:843263/1‑61 (MQ=255)ggCGCGGCAACCTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:968579/1‑61 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGTGGTTTCATTAAGCg < 1:32120/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:725700/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:1147762/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:48673/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:12536/54‑1 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCCTTCGCGGTTTCATTAAGCGGGATGa > 1:1387205/1‑53 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCgg > 1:1435050/1‑35 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1254569/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:10140/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:107095/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1122605/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1201128/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1207756/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:468434/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:919956/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:918206/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:75792/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1323183/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:446590/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:616865/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:150995/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:217697/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:300461/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:791205/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:777497/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:101051/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACAATTCGCGGTTTCATTAAGCGGGATGAg > 1:53255/1‑54 (MQ=255) | GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG > W3110S.gb/2142786‑2142864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |