| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,142,838 | 0 | A | G | 74.8% | 160.6 / 48.9 | 116 | N304N (AAT→AAC) | asmA | predicted assembly protein |
| Reads supporting (aligned to +/- strand): ref base A (24/5); new base G (86/0); total (111/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 116 total aligned reads displayed.
GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG > W3110S.gb/2142786‑2142864 | ggCGCGGCATCGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1068901/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCCCCGTTCTCACCGTTCGCTGt > 1:948447/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:387688/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:146469/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:691118/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:764676/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:1070711/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:1253271/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:92507/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg > 1:522655/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgc > 1:1052412/1‑58 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:252526/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:381014/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:326377/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:800887/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:293364/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:265200/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:454994/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:249323/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:24259/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:852649/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:166484/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:908991/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:52945/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:729353/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:705267/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:693340/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:692362/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:636359/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:735802/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:590564/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:754312/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:40128/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:518248/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:489372/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:488515/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:791211/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:729820/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:79568/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:427836/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:981995/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1134899/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1119112/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1105177/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1101798/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1100553/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1094153/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1092050/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:108963/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1470036/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1058118/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1046428/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:104485/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1029225/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1015412/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1015116/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:985917/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1157887/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1414812/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1408605/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:909452/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1378298/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1347324/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1291713/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:956311/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1252538/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1233993/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1233901/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1201575/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:959890/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1164886/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:1467305/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTAACCGTTCGCTGt > 1:374771/1‑61 (MQ=255)ggCGCGGCAACGTTGATTGGCTCAGCCCTTGCTGCGCGGCACCGTTCTCACCGTTc > 1:683260/1‑56 (MQ=255)ggCGCGGCAACGTTGATTAGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:843263/1‑61 (MQ=255)ggCGCGGCAACCTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt > 1:968579/1‑61 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGTGGTTTCATTAAGCg < 1:32120/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:1147762/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:48673/54‑1 (MQ=255) ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg < 1:12536/54‑1 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCCTTCGCGGTTTCATTAAGCGGGATGa > 1:1387205/1‑53 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:907943/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:10140/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:107095/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1201128/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1254569/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:1323183/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:919956/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:918206/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:150995/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:732154/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:217697/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:300461/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:101051/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:446590/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:468434/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:791205/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:75792/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg > 1:616865/1‑54 (MQ=255) ccccTGCTGCGCGGCACCGTTTTCACAATTCGCGGTTTCATTAAGCGGGATGAg > 1:53255/1‑54 (MQ=255) | GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG > W3110S.gb/2142786‑2142864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |