Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,162,8080GA70.9% 226.6 / 111.1 196A762A (GCG→GCAmdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base G (57/0);  new base A (139/0);  total (196/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

ATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGT  >  W3110S.gb/2162789‑2162860
                   |                                                    
ataCCTAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:174494/1‑52 (MQ=255)
ataCCCAGGACTTCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:803903/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:45722/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:638867/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:612850/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:566553/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:537667/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:524892/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:504818/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:498028/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:490231/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:485050/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:461404/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:65228/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:439982/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:411000/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:404764/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:397551/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:375284/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:290644/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:287843/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:776771/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:981413/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:946649/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:940775/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:921522/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:899539/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:858669/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:852687/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:833776/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:78268/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:357020/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:761874/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:750863/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:749219/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:714761/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:707100/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:674915/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:67308/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:667922/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1274931/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:210335/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1319449/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1203455/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1337032/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1178319/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1340817/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1352327/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1175484/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:116705/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:109579/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1214390/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1436726/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1085463/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1060452/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1234012/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1028526/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1034091/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:168091/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1047914/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1246127/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1440850/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1257463/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:160112/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:152064/1‑52 (MQ=255)
ataCCCAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1066531/1‑52 (MQ=255)
ataCACAGGACATCAGTGCACTGGAAAAAATGTTCGTTATCAATAACGAAggaa                    >  1:1420514/1‑52 (MQ=255)
          cATCAGTGCGCTGGAAGAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:322248/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAg                 >  1:1109752/1‑47 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTc    >  1:951307/1‑60 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:814623/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:7254/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:602830/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1075825/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:872180/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:907947/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:102022/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1431730/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:232981/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:246123/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:277141/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:184007/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:293047/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:31574/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:343999/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1007363/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1462089/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:419347/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:425601/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1234911/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:469878/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:478736/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1415644/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1395909/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1340036/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:552224/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1288005/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:634629/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGt  >  1:1237899/1‑62 (MQ=255)
          cATCAGTGCGCTGGAAAAAAAGTTCGTTATCAATAACGAAGGCAAAGCGAt             >  1:685986/1‑51 (MQ=255)
                   |                                                    
ATACCCAGGACATCAGTGCGCTGGAAAAAATGTTCGTTATCAATAACGAAGGCAAAGCGATCCCGCTGTCGT  >  W3110S.gb/2162789‑2162860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: