| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,414,597 | 0 | T | C | 72.5% | 124.6 / 43.9 | 98 | R293R (CGT→CGC) | acrE | cytoplasmic membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/27); new base C (71/0); total (71/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.82e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAT > W3110S.gb/3414560‑3414638 | aCCCGCAACATACGCTTTTGCC‑GGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:759441/62‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1238058/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:164574/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:160666/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1398779/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1351506/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:557896/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:133791/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:727167/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:541354/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1278401/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:277656/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1247579/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:535279/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:378884/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:961793/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1030417/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1049566/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:930688/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:8849/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:387576/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:429180/61‑1 (MQ=255) ccGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1113294/61‑1 (MQ=255) gCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:266875/59‑1 (MQ=255) cATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1091970/55‑1 (MQ=255) aTACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:1189736/54‑1 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCACCCTGACGCCATTCTTAt > 1:164311/1‑62 (MQ=38) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAAc > 1:1395122/1‑46 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGcc > 1:12885/1‑54 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:749108/1‑61 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:1118412/1‑61 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:451115/1‑61 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:1183444/1‑61 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:1211190/1‑61 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:468016/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:554605/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:456782/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:591852/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1019825/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:534321/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:529152/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:636473/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:482859/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:795289/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:949314/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:921929/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:919730/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:915980/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:892238/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:882997/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:814484/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:812605/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:657786/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:786825/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:778395/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:76639/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:742326/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:691269/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:677397/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:665516/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:664494/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1177874/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1274649/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1269849/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1263251/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1256717/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1243203/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1231205/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1223297/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1190483/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:450837/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1151300/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1149936/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1103213/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1084642/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1077028/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1065476/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1009355/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1313753/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:456288/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:381514/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:32616/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:313113/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:309118/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:258934/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:248136/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:231791/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:212284/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:167450/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:16723/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:14940/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1345487/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1340716/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1335248/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAt > 1:1299730/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGCGCGCACGGATTGATGAAGGCGTCCAACCTGACGACATTCTTAt > 1:9954/1‑62 (MQ=255) ttGCCGGGTATGTTTGTGAGCGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTa > 1:1228897/1‑61 (MQ=255) ccGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAAc < 1:859303/43‑1 (MQ=255) | ACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTAT > W3110S.gb/3414560‑3414638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |